SKC 225-9594 SKC BioSampler - Manual (Page 6)

9. Accurately measure the fi nal volume of collection liquid in an aseptic area
and aseptically transfer it to a sterilized container for analyses.
Note: If using a water-based liquid, do not sample at temperatures below
41 F (5 C) to avoid crystallization of the collection liquid.
Note: If sampling biologically inert particles, aseptic procedures are not
necessary.
Sampling with a Coated Collection Vessel
The BioSampler can collect samples into a collection vessel coated with a sticky
non-evaporating medium. Sampling times longer than 30 minutes are not recom-
mended for this method.
1. Measure and record fl ow rate, Q
s
L/min, through the BioSampler as described
on page 2.
2. Sterilize all parts of the BioSampler (see Cleaning, page 6).
3. Prepare the collection vessel by using a petroleum jelly/hexane solution
(approximately 0.1 gram petroleum jelly per milliliter of hexane). Place a few
drops of the solution into the collection vessel and rotate it so that its entire
inner surface is wett ed. Ensure that the coating covers the region where
the air jets impact the inner surface of the collection vessel. The hexane will
evaporate within fi ve minutes leaving a uniform sticky layer on the inner
surface of the collection vessel.
Note: Alternative coating methods may be used.
4. Assemble clean, sterilized inlet and nozzle sections with the prepared collec-
tion vessel in an aseptic area.
5. Warm up the vacuum pump for at least one minute before connecting it to the
sampling train (see pump operating instructions).
6. Connect outlet of newly prepared BioSampler to the inlet of a liquid trap and
the trap outlet to the vacuum pump.
7. Sample for the desired sampling period not exceeding 30 minutes.
8. Aft er sampling is complete, record the sampling time. Volume of air passed
through the BioSampler can be calculated using the formula
V [Liter] = Q
s
[L/min] x t [min].
9. Disassemble the BioSampler.
10. Aft er sampling is complete, wipe the inner surface of the collection vessel
with a small piece of cloth or paper.
11. Transfer the cloth or paper sample to a laboratory for microbiological or
chemical analysis.
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