SKC 225-9594 SKC BioSampler - Manual (Page 8)

• Immunoassay
Immunoassay is used for the quantifi cation of airborne allergens (e.g., dust
mites and animal dander). It relies on the binding of antibodies to a specifi c
target antigen. Other methods include fl uorescence, enzyme, and radioimmu-
noassay.
• Polymerase Chain Reaction
Polymerase Chain Reaction (PCR) is used to screen samples for a specifi c
genus or species. The PCR process is based on in-vitro replication of selected
nucleic acid sequences.
Note: ViaTrap mineral oil may not be suitable for PCR analysis.
References
Macher, J.M., Chatigny, M.A., and Burge, H.A., “Sampling Airborne Microorganisms and Aeroallergens,” Air
Sampling Instruments for Evaluation of Atmospheric Contaminants, 8th ed., ACGIH, Cincinnati, Ohio, 1995,
pp. 589-617
Macher, J.M., “Evaluation of Bioaerosol Sampler Performance,” Applied Occupational Environmental Hygiene,
v. 12, 1997, pp. 730-736
Buttner, M.P., Willeke, K., Grinshpun, S.A., “Sampling and Analysis of Airborne Microorganisms,” Manual of
Environmental Microbiology, ASM Press, Washington, DC, 1997, pp. 629-640
Lin, X., Willeke, K., Ulevicius, V., Grinshpun, S.A., “Effect of Sampling Time on the Collection Effi ciency of
All-Glass Impingers,” Am. Ind. Hyg. Assoc. Journal, v. 58, 1997, pp. 480-488
Willeke, K., Lin, X., and Grinshpun, S.A., “Improved Aerosol Collection by Combined Impaction and Centrifugal
Motion,” Aerosol Science and Technology, 29 (5), 1998, pp. 439-456
Lin, X., Reponen, T., Willeke, K., Grinshpun, S.A., Foarde, K.K., and Ensor, D.S., “Long Term Sampling of
Airborne Bacteria and Fungi into a Non-Evaporating Liquid,” Atmospheric Environment, 1999, pp. 4291-4298
Lin, X., Reponen, T., Willeke, K., Wang, Z., Grinshpun, S.A., and Trunov, M., “Survival of Airborne Microorgan-
isms during Swirling Aerosol Collection,” Aerosol Science and Technology, 32 (3), 2000, pp. 184-196
Willeke, K., and Macher, J. M., “Air Sampling", Bioaerosols: Assessment and Control, ACGIH, Macher, J. M.,
(editor) (1999), pp. 11-1 to 11-25
Reponen, T., Willeke, K., Grinshpun, S., and Nevalainen, A., Biological Particle Sampling. Aerosol Measurement,
Principles, Techniques, and Applications, Baron, P. Willeke, K. (eds), John Wiley & Johns, Inc, (2001), pp.
751-777
Rule, A. M., Kesavan, J., Schwab, K.J., and Buckley, T.J., “Application of Flow Cytometry for the Assessment of
Preservation and Recovery Effi ciency of Bioaerosol Samplers Spiked with Pantoea agglomerans,” Environmen-
tal Science and Technology, 41 (7), 2007, pp. 2467-2472
Nevalainen, A., Willeke K., Liebhaber F., Pastuszka J., Burge H., and Henningson E., Bioaerosol Sampling: Aero-
sol Measurement Principles, Techniques, and Applications, Van Nostrand Reinhold, New York, 1993,
pp. 471-492
7
SKC Limited Warranty and Return Policy
SKC products are subject to the SKC Limited Warranty and Return Policy, which
provides SKC’s sole liability and the buyer’s exclusive remedy. To view the com-
plete SKC Limited Warranty and Return Policy, go to htt p://www.skcinc.com/
warranty.asp.