Eppendorf UVette - Manual (Page 7)

7
Eppendorf® UVette® — Instructions for use
1. Set the parameter for the cuvette layer thickness in the device.
2. Determine the blank value of the cuvette.
Abb. 2:Absorption of the water-filled UVette
Fig. 2: Absorption of the water-filled UVette
3. Fill the cuvette with the sample.
Make sure that liquid does not spray out when solutions are mixed in the cuvette.
4. Measure the sample.
Do not use the cuvette at wavelengths below 220 nm or above 1 600 nm.
Hint!
Limited tempering efficiency!
The tempering efficiency is limited by the contour of the UVette.
•
If temperature control is required, check to see if the UVette is suitable for
your applications.
Absorbance
200 300 400
0.1
0.5
0.
Wavelength (nm)