Eppendorf R Series 48R CO2 Incubator - Manual (Page 20)

20
CO48R-0050 Š Galaxy R Series 48 R Personal Size CO
2
Incubator User’s Guide
NOTE:
Be certain to check for leaks in the CO
2
connections to avoid depleting
your CO
2
gas supply. This can be accomplished using a solution of
soapy water applied to each fitting and checking for bubbles. If any
bubbles are noted, readjust the fitting.
6. The chamber setpoints are pre-programmed at 37.0°C and 5% CO
2
. Leave the
incubator on until the programmed chamber temperature and CO
2
concentration has
been reached (please see NOTE below).
NOTE:
The incubator’s CO
2
valve is disabled until the incubator reaches the
temperature setpoint. After reaching the temperature setpoint, the CO
2
valve is activated, allowing the incubator to reach the CO
2
setpoint. If
power is interrupted to the incubator long enough for the temperature to
drop below setpoint, the CO
2
valve will be deactivated until setpoint is
again achieved. (This serves to avoid spurious CO
2
readings while the
incubator is reaching its temperature setpoint)
7. Leave the incubator running for at least two hours (preferably overnight) to allow
conditions to stabilize.
3.3 Using the Humidity Tray & Humidity Control
If humidification is required, the humidity tray should be filled with 0.5 liters of warm
(
∼ 37.0ºC) distilled water at this time.
For cell culture work, we recommend the use of copper sulphate (or a recognized
biocide) in the humidity tray. Tests have shown that, in addition to inhibiting bacterial
growth in the tray, this can reduce contamination on the chamber walls. Add one small
teaspoonful (~0.11 oz or 3.2 g) of copper sulphate each time you fill the tray with fresh
water.
For IVF and other sensitive work, we do not recommend the use of any biocide in the
humidity tray. To reduce the possibility of contamination, every 10 to 14 days, empty
the tray, clean it with a solution of 70% isopropyl alcohol and 30% distilled water, and
then refill it with 0.5 liters of warm distilled water.
To remove the humidity tray, turn it 45º to clear the shelf rack supports (see Figure 3a).