Eppendorf R Series 48R CO2 Incubator - Manual (Page 48)

48
CO48R-0050 Š Galaxy R Series 48 R Personal Size CO
2
Incubator User’s Guide
Set up the nitrogen tanks as follows:
1. Inspect them to be sure there are no leaks or other damage.
2. Check that the two-stage pressure regulator valve and the inline pressure
regulator valve are closed by trying to turn their knobs in a counter-
clockwise direction; the knobs should be at the stop point.
3. Place the inline pressure regulator close to the incubator.
4. Securely attach the two-stage pressure regulator to the nitrogen tank’s outlet
and to the inline pressure regulator’s inlet with tubing, securing both ends
with clips.
5. Connect the inline pressure regulator’s outlet to the incubator’s N
2
inlet
located at the top middle of the incubator’s rear panel; secure both ends with
clips.
6. Proceed as indicated in Section 8.5.2.
NOTE:
To add an automatic gas cylinder changeover instrument: with the
incubator turned off, connect its inlet to the two-stage pressure
regulator’s outlet, and the changeover instrument’s outlet to the inline
pressure regulator’s inlet with tubing, securing the ends with tubing clips.
8.5.2 Setting Up Oxygen Control
1. Remove the black plastic protective cover from the hydrophobic filter cap
(making sure that the hydrophobic filter cap is not removed with it), located
in the rear wall of the incubator chamber. Retain the cover for use when you
clean the chamber.
2. Open the nitrogen gas supply from the tank. Set the N
2
tank’s outlet
pressure gauge to 1.5 bar.
3. Set the nitrogen regulator to 1 bar (14.5 psi). The nitrogen will be fed into
the incubator through a HEPA filter that is already installed in the gas line.
The gas flow rate is approximately 20 liters/minute.
NOTE:
If the programmed O
2
level is close to the ambient oxygen, it may be
necessary to reduce the cylinder pressure below 1 bar to stop the oxygen
level from undershooting the programmed value.