C.B.S. Scientific DASG-250-02 - Manual (Page 13)

www.cbsscientific.com 13 Adjustable Slab Gel Manual 07/13
3.4 Running the Gel
1. Attach plate set(s) to unit with
smaller or notched plate facing
towards the upper buffer reservoir. If
using a pre-cast gel stored at 4°C,
allow to warm to room temperature.
Use white clamps with mouth open
(cat. # GPC-0001) to attach plate
set to unit, clamping each side to
the sandwich.
1b.
Optional Cooling Plate
Instructions:
If using optional aluminum plate
during electrophoresis place behind
the unit shown in figure at right.
Secure in place with 2 each GPC-
0001
white clamps (ordered
separately).
2. Pour freshly prepared buffer in
upper and lower chamber. Using a
pipette or syringe, thoroughly flush
out the wells in the glass plate
sandwich with buffer from the upper
chamber. Boil or heat samples and
then place immediately on ice. Load
samples. If outer lanes do not
contain sample, it is recommended
that you run standards (Rainbow™
standards can be monitored easily
during run) and/or fill outer lanes
with loading buffer to reduce smiling
and wrap-around effects.
3. Attach safety cover.
4. Connect the leads to the power
supply, matching the color-coded red
to red and black to black. See
Section 4.1 for recommended
power conditions. Begin
separation by electrophoresis