C.B.S. Scientific LDASG-400-20 - Manual (Page 10)

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4. Place the notched plate on top of the bottom
assembly, starting from the bottom edge and
gently easing the plate down. Verify the gasket is
smooth around the edges and then clamp along
the bottom.
5. Lift the assembly and stand it on the base of the
clamps. For leveling, push glass plate assembly
down until it stops against clamp body. Clamp
the sides of the assembly with additional casting
clamps on either side. As each clamp is
attached, be sure the gasket is aligned between
the plates forming a seal.
6. Apply PAGE or agarose solution to gel plate sandwich using a syringe or pipette. If
using a stacking gel, pour desired height of running gel, then overlay a small amount
of dH
2
O or 0.1% SDS solution to top of gel. After polymerization, rinse with buffer,
add stacking gel solution and insert comb. For regular, unit percentage gels, add
polyacrylamide solution to correct height, and insert comb. Allow gel to set, usually
20 minutes. Extra gel solution in pipette or syringe can be monitored to test
polymerization of gel mix.
7. Disassembly. Hold the clamped plate
assembly with one hand. Remove the gasket
by starting at one of the top ends and pulling up
and out on the gasket until it releases from the
plate, up to the bottom of each of the white
clamps. When each clamp is reached DO NOT
remove it, instead feed the gasket down
through the clamp body and repeat pulling up
and out. Continue feeding until the gasket is
fully detached. If gel, is not to be used
immediately, wrap entire plate sandwich with
plastic wrap tightly to seal and store at 4°C for
up to a month.