Leica Biosystems PELORIS_PELORIS II - Manual (Page 141)

Troubleshooting
Leica PELORIS™ User Manual Rev K © Leica Biosystems Melbourne Pty Ltd 2011 141
9.2.1 Under-processed Tissue – Instrument Setup
Under-processed tissue
(soft/mushy/wet)
Yes
Yes
No
No
No
No
Yes
No
Stop:
well processed tissue
Yes
Yes
Go to 9.2.3 Under- or Over-
processed Tissue – Reagents
No
Are any
error codes
present?
If a user warning, fix the
issue. If a service warning,
contact service
Retest.
Is the tissue
processed
correctly?
Was the
correct protocol
used?
Check:
tissue size
step times
pressure and vacuum
temperatures
drip times
Are the settings appropriate?
Optimize the reagent using the predefined
protocols as a guideline
Was the
correct carryover
value, number of
cassettes,
entered?
Enter correct values for carryover and
cassettes for the reagent management
system to work correctly.
Retest.
Is the tissue
processed
correctly?
Retest.
Is the tissue
processed
correctly?
Is the lab
workflow
appropriate?
Check:
Transfer fluid (formal saline?)
Fixation time
Water introduced after processing?
Quality of embedding wax
Are these all correct?
No
Retest.
Is the tissue
processed
correctly?
Yes
Yes
Yes
No