UVP HB-500 Minidizer - Manual (Page 15)

HB-500 Minidizer Hybridization Oven 15
Protocol 3:
Chemiluminescence Detection: HRP-Tagged, Alkaline Phosphatase (AP) Probes
or Antibody Conjugates
Equipment
•
Clear plastic cling-wrap or Clear transparent sheet protector
•
UVP EC3 or AC1 Darkroom with Cooled CCD camera
•
Pipette
Reagents for Chemiluminescence
•
ECL™ (or other) detection reagent 1
•
ECL™ (or other if required) detection reagent 2
•
Membrane following hybridization.
Procedure
1. Mix equal volumes of detection reagents 1 and 2
2. Pipette the mixture over the surface of the membrane and leave at room temperature for 1 minute.
3. Drain the sample and wrap it transfer to Darkroom
4. Close the darkroom and image using CCD camera.
References
Ross, J; Nucleic Acid Hybridization: Essential Techniques; 1998, John Wiley and Sons, ISBN 0-471-97125-1
Sambrook, J; Molecular cloning: a Laboratory manual; 1987, Cold Spring Harbor Laboratory Press; ISBN 0-87969-
309-6
Current Protocols in Molecular Biology; 1987, John Wiley and Sons; ISBN 0-471-50338-X