UVP HB-500 Minidizer - Manual (Page 3)

HB-500 Minidizer Hybridization Oven 3
Introduction
The HB-500 Minidizer Hybridization Oven is a cost-effective, personal desktop hybridization oven with a capacity of
four 35x150mm bottles, eight 50ml or 15ml conical plastic tubes. The Minidizer operates with a state-of-the-art
microprocessor as well as temperature and variable speed controls. The HB-500 Minidizer enables consistent
saturation of samples, whether it for washing or hybridizing. The bottle carousel rotates at a uniform speed of 12
RPM.
A touch-sensitive keypad and microprocessor provide accurate temperature control from ambient +10°C to 78°C. The
keypad is located below a large LCD readout. Once the desired hybridization temperature is entered, the readout
displays the current temperature inside the chamber. The chamber environment is calibrated at 78°C; at this
temperature, the microprocessor accuracy is ±0.5°C. The uniformity of the chamber at 68°C is ±0.1°C; at 55°C is
±0.1°C; and at 42°C is ±0.1°C.
WARNING!
There may be build up of pressure within the hybridization bottles when they are taken from ambient to hybridization
temperature. To help in relieving some of this pressure, preheat the solutions and bottles. Also, ensure that bottles
are opened at the same temperature as the hybridization process. Do not allow the bottles to cool before opening.
To assure hybridization bottles remain leak proof and pressure proof, prevent temperatures above 70°C. If
temperatures above this are used without first relieving the pressure within the bottle, there is a risk that bottles will
leak and/or break due to internal pressure build up.
If the bottles are accidentally taken above 70°C without relieving the pressure, DO NOT open the hybridizer door.
Turn off the hybridizer and allow it to cool before opening the door and checking the contents of the oven. Should the
bottles be used above 70°C, relieve the pressure by unscrewing and re-tightening the cap.
If using radioactive material see the “Decontamination” section of this manual under “Service Procedures”.