UVP BioSpectrum Imaging System - Manual (Page 18)

BioSpectrum Imaging System 18
Camera Control
The camera is controlled via VisionWorksLS software by clicking on the
Acquisition Action Tab and then clicking on the Camera Menu Button.
(Cameras have different functionality. Some options listed to the right in
the Camera Menu may not appear for each camera. VisionWorksLS
software automatically detects and enables the built-in functionality for
each camera.)
Integration: Integration allows the user to automatically capture multiple
images, whether at regular intervals or at user-defined intervals.
Integration also allows the user to stack multiple images into one or to
save each image separately.
Exposure Time: Use the various sliders and drop-downs to select the
ideal exposure time for each specific experiment.
Binning: Use binning to achieve quicker preview and capture images.
CCD Temperature: When using cooled cameras for longer exposures
(such as with chemiluminescent experiments), the CCD temperature
status area will inform the user when the CCD’s temperature is low
enough for long exposure times.
Lighting and Emission Filter Selection
The lighting in the BioSpectrum darkroom is controlled via the
Lighting & Filters section of the Lighting Menu in VisionWorksLS.
1. Select the appropriate emission Filter from the drop-down
menu.
2. Select the appropriate Epi Illumination source from the
buttons.
Plates cover the lights to prevent any reflective light when
lighting is not required. When illuminating the epi lighting,
remove the plates and store in a convenient place to reattach
when needed.
3. Select the appropriate Transillumination source from the buttons.
When using transillumination, switch the transilluminator power to ON (switch is located on the
front of the transilluminator).
When using the LED White Light Illuminator, ensure that it is plugged in to the power receptacle
located at the upper rear right corner of the darkroom.
Note: The UV transilluminator will automatically shut off when the darkroom door is open. The
transilluminator also has a timer to shut off the transilluminator after 20 minutes of inactivity.
Note: The BioSpectrum includes a UV interlock switch that shuts off all UV operation when the
darkroom door is opened. Be sure the darkroom door is completely closed to ensure proper UV
operation.