UVP ChemiDoc-It 2 Imagers - Manual (Page 19)

GelDoc-It
2
and ChemiDoc-It
2
Imagers 19
To Process a Blot
1. Place the Western blot on a hinged transparency or in clear plastic bag.
2. Position the blot on the black chemi tray.
3. Pre-focus and position the blot with the lights off, the door cracked open and the lens set to the
fastest f-stop (the lowest aperture number). Use the “Chemi Blot Pre-Capture Positioning”
template discussed earlier.
4. Apply the chemi substrate on the blot (front side up). Then, close the hinged transparency over
the blot and smooth out any bubbles. Reposition the blot in the field of view if needed. Close the
darkroom door.
5. Immediately start previewing with the “Chemi Blot with Fast Capture and Lower Resolution”
template. Let the blot develop inside the darkroom while watching the preview. When
approaching maximum signal (which should take 2 to 3 minutes) use the “Chemi Blot with Lower
Capture and Higher Resolution” template to capture the image.
6. Light emission will drop off quickly, so capture several images as quickly as possible.
7. Save and archive original files for future quantitative analysis.
8. After creating a copy of an image file, use the image invert function in VisionWorksLS, adjust the
image histogram for optimal viewing, and burn the changes to a new file.
Note: Capture and image processing steps are described in more detail in the LS software manual
and in the software help files.