UVP ChemiDoc-It 2 Imagers - Manual (Page 17)

GelDoc-It
2
and ChemiDoc-It
2
Imagers 17
Capturing Images and Using Templates
Image Focusing
Manual Lens (GelCam 310 with Manual Lens)
Prior to capturing an image, prepare the image focus:
1. Turn on the transilluminator and place the Fluorescent Focus Target (see “System Components”)
on the transilluminator surface. NOTE: The darkroom has a UV safety switch that turns off the
transilluminator and optional UV handheld lamps when the door is open. After closing the door,
open the UV Gel Viewer Window to ensure that the transilluminator is on and that all other lighting
is off.
2. With VisionWorksLS open, preview the image. Adjust the camera settings, including exposure time,
to enhance the image of the sample. To adjust the settings, go to the Acquisition Action Tab and
click on the Camera Menu Button. Adjust the exposure time from the Exposure Time section of the
Camera Menu.
3. Rotate the lens f-stop adjustment (top ring) so that the image is bright enough to be seen on the
screen.
4. Rotate the lens focus adjustment (bottom ring) on the lens. Adjust the focus so that the image
appears clear on the screen.
5. Rotate the zoom lens adjustment (middle ring) on the lens so that the image is ideally zoomed.
Readjust the focus ring (bottom ring) on the lens, making the image clear. Adjust the zoom so that
the object of interest is within the image preview area.
Motorized Lens (GelCam310 w/Motorized Lens, MegaCam 810, OptiChemi 610 and
BioChemi 510)
Prior to capturing an image, prepare the image focus:
1. Turn on the transilluminator and place the Fluorescent Focus Target (see “System Components”)
on the transilluminator surface. NOTE: The darkroom has a UV safety switch that turns the
transilluminator and optional UV handheld lamps off when the door is open. After closing the door,
open the UV Gel Viewer Window to ensure that the transilluminator is on and that all other lighting
is off.
2. Open VisionWorksLS software, click on the Acquisition Action Tab, and then click on the Lighting
Menu Button. Adjust the aperture, zoom (if applicable) and focus controls from the Lens Control
section of the Lighting Menu until an ideal image is seen.
3. Adjust the camera settings, including exposure time, to enhance the image of the sample. To
adjust the settings, go to the Acquisition Action Tab and click on the Camera Menu Button. Adjust
the exposure time from the Exposure Time section of the Camera Menu.
Capturing Images
1. Depending on the sample type, place the sample on the chemi tray, transilluminator or white light
plate.
2. For a chemiluminescent sample, turn off all darkroom lighting, place the black chemi tray on top of
the transilluminator and place the sample on the tray. For a fluorescent gel sample, turn on the UV
transilluminator.
3. Adjust the capture settings in VisionWorksLS.
4. Click the Capture button.