UVP ChemiDoc-It 2 Imagers - Manual (Page 5)

GelDoc-It
2
and ChemiDoc-It
2
Imagers 5
Cameras
GelDoc-It 2 : The GelDoc-It 2 is equipped with the GelCam 310 camera, a scientific-grade monochrome
CCD camera with a resolution of 2.0MP (1600x1200) with USB 2.0 PC interface.
ChemiDoc-It
2
: The ChemiDoc-It
2
is equipped with the BioChemi™ 510, OptiChemi™ 610 or
MegaCam 810 scientific-grade monochrome CCD camera. All cameras are Peltier cooled and offer full
16-bit file bit depth:
The BioChemi 510 camera has 2.1MP resolution with Peltier cooling to -35°C from ambient. The
BioChemi 510 has a peak quantum efficiency of 50% and is capable of binning from 1x1 to 8x8.
The OptiChemi 610 camera has 3.2MP (2184x1472) resolution with Peltier cooling to -50°C from
ambient. The OptiChemi 610 has a peak quantum efficiency of 86% and is capable of binning from
1x1 to 10x10.
The MegaCam 810 camera has 8.1 MP (3296x2472) resolution with Peltier cooling to -35°C from
ambient. The MegaCam 810 has a peak quantum efficiency of 50% and is also capable of binning
from 1x1 to 8x8.
All camera settings are factory pre-set for optimum performance when viewing gels, films or
membranes under low light level conditions. Contact UVP Technical Support prior to making any
adjustments to the camera settings.
Lens Kit (Motorized or Manual)
The zoom lens used with the GelCam 310 and BioChemi 510 cameras comes in two configurations:

The motorized lens is controlled via VisionWorksLS Software.

The manual lens requires manual user adjustment.
Some lenses may be fitted with a close-up diopter. The diopter is used for focusing on objects at the
focal length of the GelDoc-It
2
/ChemiDoc-It
2
darkroom.
Ethidium Bromide (EtBr) Emission Filter
The ethidium bromide (50mm square) UV-blocking bandpass interference filter blocks UV and IR
radiation emitted from the transilluminator. The filter is placed in the filter wheel below the camera
assembly. The filter allows visualization of fluorophores from 580-630nm, targeting the ethidium
bromide emission peak of 605nm.
Additional filters are available for other specific fluorophores. Filters can also be removed when imaging
non-fluorescent media (including chemiluminescent blots, protein gels, colony plates, etc.) in order to
produce brighter images. Contact UVP for ordering information.
Darkroom
The darkroom is light tight to provide optimal imaging conditions. Darkroom features include:

Overhead white light

Roll-out transilluminator tray

UV-safe gel viewer window built into the darkroom door

Side pocket for storage of materials

Four position emission filter wheel

Brackets for installing optional UV handheld lamps for epi (overhead) UV illumination

Built-in power ports for transilluminator and optional overhead UV handheld lamps

Built-in power cord for optional LED White Light Plate

UV safety interlock switch with manual override to disable UV transillumination and
optional UV epi illumination when darkroom door is opened