Waters XSelect CSH HPLC Columns - Manual

[ CARE AND USE MANUAL ]
CONT ENTS
I. GET TING START ED
a. Column Installation
b. Column Equilibration
c. Initial Column Efficiency Determination
II. COLUMN USE
a. Guard Columns
b. Sample Preparation
c. Operating pH Limits
d. Solvents
e. Pressure
f. Temperature
III. SCALING UP/DOWN ISOC RATIC MET HODS
IV. T ROUBLESHOOTING
V. COLUMN CLEANING, REGENERATION
AND STORAGE
a. Cleaning and Regeneration
b. Storage
VI. CONNECTING T HE COLUMN TO T HE HPLC
a. Column Connectors and System Tubing Considerations
b. Measuring System Band-spreading Volume and System Variance
c. Measuring Gradient Delay Volume (or Dwell Volume)
VII. ADDITIONAL INFORMATION
a. Use of Narrow-Bore (3.0 mm i.d.) Columns
b. Impact of Band-spreading Volume on 2.1 mm i.d.
Column Performance
c. Non-Optimized vs. Optimized LC/MS/MS System:
System Modification Recommendations
XSELECT CHARGED SURFACE HYBRID (CSH) COLUMNS
Thank you for choosing a Waters XSelect ® CSH column. This HPLC
column features Waters Charged Surface Hybrid (CSH) Technology
which provides excellent peak shape, high efficiency and loading
capacity for basic compounds when using low ionic strength, acidic
mobile phases. This same particle technology is used in the ACQUITY
UPLC
® CSH column family, thus enabling seamless transferability
between HPLC and UPLC ® system platforms. All XSelect CSH packing
materials are manufactured in a cGMP, ISO 9001:2000 certified plant
using ultra pure reagents. Each batch of XSelect CSH material is
tested chromatographically with acidic, basic and neutral analytes
and the results are held to narrow specification ranges to assure
excellent, reproducible performance. Every column is individually
tested and a Performance Test Chromatogram is provided with
each column along with the Certificate of Acceptance.