Waters Peptide Separation Technology ACQUITY UPLC BEH130 and BEH300 - Manual

[ CARE AND USE MANUAL ]
CONT ENTS
I. INT RODUCTION
II. GET TING START ED
a. Column Connectors
b. Column Installation
c. Column Equilibration
d. eCord Installation
e. Initial Column Efficiency Determination
III. COLUMN USE
a. Sample Preparation
b. pH Range
c. Solvents
d. Pressure
e. Temperature
IV. COLUMN CLEANING, REGENERATING
AND STORAGE
a. Cleaning and Regeneration
b. Storage
V. INT RODUCING eCORD INT ELLIGENT
CHIP T ECHNOLOGY
VI. REP RESENTATIV E T EST CHROMATOGRAPH
AND CONDITIONS FOR SEPARATION OF
P ROT EIN DIGEST
ACQUITY UPLC Peptide BEH C
18
, 130Å and 300Å Columns
I. INT RODUCTION
Thank you for choosing an ACQUITY UPLC Peptide BEH C
18
, 130Å or
300Å Column. The ACQUITY UPLC Peptide BEH C
18
, 130Å and 300Å
packing materials were designed specifically for use with the Waters
ACQUITY UPLC System and are manufactured in a cGMP, ISO 9002
certified plant using ultra pure reagents. Each batch of ACQUITY
UPLC BEH material is tested chromatographically with acidic, basic
and neutral analytes and has been qualitifed for peptide mapping.
The results are held to narrow specification ranges to assure
excellent, reproducible performance. Every column is individually
tested and a Performance Chromatogram and Certificate of Batch
Analysis are provided on the eCord™ intelligent chip. Each batch of
ACQUITY UPLC Peptide BEH C
18
, 130Å and 300Å Columns is also QC
tested with the separation of a protein digest.