Waters NanoEase Trapping Columns - Manual (Page 2)

[ Care and Use ManUal ]
NanoEase Trapping Columns 2
4. To secure the column into a multi-port valve, care must be taken
to get the face of the stainless steel barrel seated properly into
the port without introducing dead volume between the frit at the
end of the stainless steel barrel and the valve port. The stainless
steel barrel must not protrude too far past the end of the black
fitting, otherwise there will not be enough threads of the fitting
protruding into the valve port to make a proper seal. Consult Fig-
ures to see how to properly seal the trap into the valve port and
connect the desired tubing. The following steps are suggested:
a) First make sure that the trap column insert (Figure 2) is
inside the black fitting (Figure 1b).
b) Screw in a column-blanking plug into the top of the black
fitting (Figure 3) until the stainless steel barrel protrudes
from the end of the fitting approximately between 2 and 3
mm (Figure C). It may be necessary to use your finger (use
a glove to avoid contamination of the trap) to push against
the end of the stainless steel barrel so that the other end of
the insert is set against the column-blanking plug and the
stainless steel barrel protrudes from the end of the black
fitting at the proper depth. Adjust the position of the col-
umn-blanking plug if necessary.
a. Illustration of adjusting the length of the stainless
steel barrel
Figure A. Incorrect. Results in dead volume between valve port and trap column
Figure B. Incorrect. Note these are insufficient number of threads to seal trap
column to valve
Figure C. Correct.
2-3 mm
Figure 1a
Figure 3
Figure 1b Figure 2