Waters NanoEase Trapping Columns User Manual

Care and use manual, Nanoease trapping columns

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[ Care and Use ManUal ]

NanoEase Trapping Columns

1

i. introduction:

Waters NanoEase Trapping Columns are robust devices that can effectively
separate complex samples and remove buffers and high concentrations of
salts. For the latest on Life Science Chemistries, go to:
www.waters.com/lifesciences.

ii. nanoEasE trap column prEparation

1. It is recommended that the trap column be washed and conditioned

with a suitable solvent before use. This can be accomplished using
a suitable syringe to flush solvent through the column. If a syringe
is used for this purpose, it is recommended that a syringe with an
inert teflon plunger and glass body be used to avoid contami-
nation. Syringes with rubber plungers or plastic bodies should
not be used. Alternatively, the trap column can be placed on the
HPLC system and washed with suitable solvent at a flow rate of
10-40 µL/min for several minutes prior to use.

2. If using a 10-port valve, use UpChurch

®

long nuts (UpChurch

part # F-285X) in each adjacent port of the 10-port valve, due to
the clearance width of the trap column.

3. The trap column works with the black 10-32 outer fitting with

white label (Figures 1a and 1b), which secures the stainless steel
tube and the tan PEEK

®

(column insert part) piece (Figure 2) to the

switching valve.

contEnts

i. introduction

ii. nanoEasE trap column prEparation

iii. GEnEral GuidElinEs for column usE

iv. ordErinG information

nanoEasE trappinG columns

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