Metrohm 940 Professional IC Vario ONE/SeS - Manual (Page 43)

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4 Installation
940 Professional IC Vario ONE/SeS (2.940.1400)
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￭ Rinse the separation column with eluent for approximately 10
minutes.
￭ Use manual control in MagIC Net to stop the high-pressure pump
again: Off.
4.21 Conditioning
After installation, after each time the instrument is switched on and after
each time the eluent is changed, the system has to be conditioned with
eluent long enough that a stable baseline is attained.
NOTE
The conditioning time can lengthen considerably after changing the
eluent.
Conditioning the system
1 Preparing the software
CAUTION
Ensure that the configured flow is not higher than the flow permit-
ted for the corresponding column (refer to the column leaflet and
the chip data record).
￭ Start the MagIC Net computer program.
￭ Open the Equilibration tab in MagIC Net: Work-
place
▶ Run ▶ Equilibration.
￭ Select (or create) a suitable method.
Also see: MagIC Net tutorial and online help.
2 Preparing the instrument
￭ Ensure that the column is inserted correctly in relation to the flow
direction marked on the sticker (arrow has to point in the direc-
tion of flow).
￭ Ensure that the eluent aspiration tubing is immersed in the eluent
and there is enough eluent in the eluent bottle.
3 Starting equilibration
￭ Start the equilibration in MagIC Net: Workplace ▶ Run ▶ Equi-
libration ▶ Start HW.