Bio-Rad Gel Doc™ XR+ System - Manual (Page 2)

IPD Multi-Color Imaging Target
Please find attached Bio-Rad’s new Multi-Color Imaging Target and suggested protocol. The
Multi-Color Imaging Target has been designed for use with all IPD instruments. The target was
developed so that you can quickly demonstrate the performance capabilities of all IPD
instruments without a biological sample. THIS TARGET IS NOT INTENDED FOR
QUANTITATIVE (QC) USE BUT RATHER AS A QUALITATIVE TOOL. It can be
applied for some general quantitation, but the inks are not uniformly printed and will yield
variable results. The main advantage of the imaging target is that you may no longer need to
have the customer run samples in order to acquire a quick image and show off the system. This
target supports 4 different imaging modes (psuedo-applications) allowing reps. to show
customers various features of the system being demonstrated.
I. Green-Fluorescence (emission peak at 534 nm)
•
single channel fluorescence can be shown using the FITC or other green
fluorescence applications on the Fluor-S, Fluor-S MAX or Molecular Imager
FX.
•
multi-color fluorescence can be shown by first scanning with a green
application and then scanning with a red application using the Fluor-S or
Fluor-S MAX. For the FX, both the red and green will be excited and exhibit
fluorescence using either the 488 or 635 nm laser lines.
•
uniformity of fluorescence image collection can also be shown (qualitatively)
by doing a profile (or volume box) analysis on all green squares after
acquisition.
•
NOTE: The ChemiDoc and Gel Doc systems will show both red and green
fluorescence with the EtBr filter.
II. Red-Fluorescence (emission peak at 637 nm)
•
single channel fluorescence can be shown using the Texas Red application.
•
multi-channel fluorescence can be shown in scanning first with Texas Red
and then switching to the FITC or other green fluorescence application.
•
fluorescence image collection can also be shown (qualitatively) by doing a
profile analysis on all red squares after acquisition.
•
customers can use the fluorescent red ruler for gel measurements and
alignment.
•
NOTE: The ChemiDoc and Gel Doc systems will show both red and green
fluorescence with the EtBr filter
III. Phosphorescence (chemiluminescent applications)
•
phosphorescence can be shown using the chemiluminescence mode(s).
•
relative lens uniformity can also be shown using profile (or volume box)
analysis of all the square blocks.
IV. Resolution (colorimetric applications)
•
resolution scanning can be shown using profile and zoom analysis of 4-set
line sequences on all IPD systems.