Bio-Rad Gel Doc™ XR+ System - Manual (Page 3)

Imaging Target Protocol
The following protocol is recommended for the Multi-Color Imaging Target. The target should
be used for qualitative, not quantitative (QC) analysis.
I. For Chemiluminescence
a) Place target in light free (dark) environment for 2 minutes. Target can be placed inside
instrument or covered with a dark material to accomplish this.
b) Remove target from dark environment and expose to fluorescent white light for 2 minutes by
lying target flat perpendicular to light source. When using a fluorescent white light source
place the target directly against source. For fluorescent room lighting place target on
tabletop.
c) Quickly place target in imaging instrument and center using “position” and/or “focus”. The
target’s outer most four white-colored corners should be seen in entire image. Then close
instrument door. Wait for 20 seconds.
d) Following the 20 seconds, acquire a 5 second image using appropriate application(s).
Analysis:
Uniformity can be done using the profile or volume box tools in Quantity One.
II. For Fluorescence
a) Place target on platen, center and focus so that the target’s outer most four white-colored
corners are seen in entire image.
b) Acquire image using either trans-UV or epi-UV (or laser line) using the appropriate
fluorescent application.
Analysis:
Uniformity can be done using the profile or volume box tools in Quantity One.
III. For Densitometric Analysis
a) Center and focus the imaging target on the instrument platen.
b) Select the appropriate application (x-ray film)
c) Use either trans or epi white light for illumination and image capture.
Analysis:
Resolution can be shown by zooming in to the 4-line segments of the imaging target.