Bio-Rad Profinia Protein Purification System Buffers and Reagents - Manual (Page 2)

Specifications
Quantity 45 g, 90 g
Description Profinia urea packs contain
high-purity chromatography
grade urea which is ideal for
solubilizing and denaturing
proteins in E. coli extracts.
Storage Store between 4°C and 22°C
Instructions Add the entire contents of the
for use 90 g pack to the 176 ml of
Profinia Denaturing IMAC
Lysis Buffer (#620-0209) or
Profinia Denaturing IMAC
Wash Buffer 1 (#620-0210) to
make 250 ml of a 6 M urea
solution. Add the entire contents
of the 45 g pack to the 88 ml of
Profinia Denaturing IMAC
Wash Buffer 2 (#620-0211) or
Profinia Denaturing IMAC
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