Bio-Rad Profinia Protein Purification System Buffers and Reagents - Manual (Page 3)

Elution Buffer (#620-0212) to
make 125 ml of a 6 M urea
solution. Stir to dissolve, and
filter through a 0.45 µm filter to
remove any particulates. Fresh
urea solutions should be prepared
and used the day of
chromatography. Unused
solutions can be stored at 4°C
and be used within 7 days, or
stored at –20°C for up to 6
months and thawed once prior
to use.
Shelf life The urea packs are stable for at
least 2 years from date of
receipt. Refer to the specific
expiration date on the product
label.
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