Bio-Rad Aurum™ Total RNA 96 Kit - Manual (Page 19)

15
Section 9
Troubleshooting Guide
Problem Possible Cause Recommended Solution
Difficulty achieving Purge step in protocol If sealing of wells with
–17 to –23 inHg sealing tape restores
negative pressure negative pressure, no
corrective action is
required; however, do
not leave sealed
Open vacuum regulator Turn control knob fully
clockwise to close
regulator
Plate not seating on Press down gently on
vacuum manifold RNA binding plate to
seat
Manifold top not seated Ensure that manifold
properly on base top is seated properly on
base
Residue on gasket Rinse manifold and
gasket with distilled
water and wipe dry
Gasket detached from Press down to
manifold reestablish uniform
adhesive contact or
replace gasket
Worn manifold gasket Replace gasket
Cross-contamination Abrupt application of Increase negative
between adjacent negative pressure during pressure gradually
wells elution over 5–10 sec using
the vacuum regulator
Residual wash buffer on Blot RNA binding plate
drip directors drip directors with paper
towels; ensure that the
plate underside is dry