Bio-Rad Aurum™ Total RNA 96 Kit - Manual (Page 6)

Section 3
Storage Conditions
All kit components (including lyophilized DNase I) should be stored at
room temperature. Store reconstituted DNase I at –20°C in a nonfrost-
free freezer, avoiding repeated freeze-thaw cycles. If precipitation is
observed in any solution, warm the solution to 37°C to redissolve and
allow the solution to return to room temperature before use.
Section 4
Necessary Supplies
Equipment and supplies to be provided by the customer:
• Plate centrifuge, capable of generating 1,500 x g
• b-mercaptoethanol, 14.2 M (catalog # 161-0710)
• Lyticase (for yeast RNA isolation only)
• Lysozyme (for bacterial RNA isolation only)
• Isopropanol (for bacterial RNA isolation only)
• 95–100% ethanol
• Tris for DNase I reconstitution (catalog # 161-0716)
• Multichannel pipettor (recommended)
• Reagent reservoirs (recommended)
• Additional sealing tape (catalog # MSB-1001) (optional)
Additional equipment required for vaccum format:
• Aurum™ Vacuum Manifold with regulator (catalog # 732-6470)
• Vacuum source (capability of –23 inHg or approximately –800 mbar
required)
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