Bio-Rad ReadyPrep™ Reduction-Alkylation Kit - Manual (Page 10)

Section 5
Appendix
5.1 2-D Rehydration/Sample Buffer Volume
The table that follows indicates appropriate volumes of 2-D
rehydration/sample buffer needed to rehydrate IPG strips of
specific lengths and the approximate amounts of protein
required for detection using silver stain or Coomassie Blue
G-250 stain. When loading the protein onto the IPG strips
by cup loading, refer to the Cup Loading Tray instruction
manual (4006216) for detailed instructions. When loading
the protein onto the IPG strips by passive/active rehyrdra-
tion, refer to the ReadyStrip
™ IPG Strip instruction manual
(4006166) for detailed instructions.
IPG strip length 7 cm 11 cm 17 cm 18 cm 24 cm
Rehydration 135 µl 200 µl 330 µl 345 µl 450 µl
volume-cup loading
Rehydration 125 µl 185 µl 300 µl 315 µl 410 µl
volume–passive/
active rehyrdration
Protein load– 5–20 µg 20–50 µg 50–80 µg 50–80 µg 80–150 µg
Silver stain
Protein load– 50–100 µg 100–200 µg 200–400 µg 200–400 µg 400–800 µg
Coomassie G-250
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