Bio-Rad ReadyPrep™ Reduction-Alkylation Kit - Manual (Page 14)

complete 2-D rehydration/sample buffer, add 1.1 ml of PSB
diluent to each 1 g of PSB powder.
Note: Before weighing out the PSB powder, shake the bottle
vigorously for 10–15 sec to break up any clumps and to ensure a
uniform blend of the different components.
Mix the solution until the powder is completely dissolved
(the tube can be warmed to speed dissolution of the solids,
but do not allow the temperature to exceed 30°C). Add
Bio-Lyte ampholyte and Bromophenol Blue as directed in
Section 5.2.2.1 to complete the preparation of the buffer.
Do not add reducing agent.
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