BUCHI Fraction Collector C-660 User Manual

Page 27

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6 Operation

27

C-660 Operation Manual, Version F

Table 6-9: Parameters for configurationm (cont.)

Parameter

Description

Sensitivity

The adjustment of various switching sensitivities. Range 4 shows the most sensitive,
Range 1 the most sluggish switching behavior.

This function enables adjustment of the collection function to the relevant slope of the
peak. Peaks with short elution times have steep flanks and the switching decision must
be correspondingly quick. Conversely, peaks with longer elution times have considerably
flatter flanks and are usually somewhat more agitated; such peaks require a more slug-
gish switching decision. The following serves as a rule of thumb:

- Use Range 3 for separations on small columns with diameters of 12...15, range 2 for

larger columns.

- Switch over to the next lower range if the peak detector switches too often. Switch

over to the next highest range if it switches too sluggishly or ‘overlooks’ unambiguous
switching criteria.

Min.

Min.

0

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

time (min)

si

g

n

al

(

1000 m

V

=

100%

)

min

min

min

max

max

max

max

threshold

min

10 %

min

Fig. 6.7: Example diagram of a detection after peak minimum (min) and peak maximum (max) with a threshold of 10%.

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