Hoefer PCR1000 - Manual (Page 12)

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Using the PCR Workstation
Introduction
The Hoefer ® PCR Workstation provides effective
decontamination of reagents and equipment before
carrying out sensitive PCR
reactions, particularly
when amplifying DNA sequences which are either in
limited supply or of low copy number. Four timer-
controlled 16-Watt UV bulbs enable the user to
control the exposure time and dose of high energy
UV irradiation required to denature nucleic acids that
cause unwanted background contamination, while a
timer override switch allows the UV source to remain
on indefinitely if preferred. Safety interlocks switch-
off the UV bulbs automatically when the cabinet side
doors are opened, preventing accidental exposure
to the UV source. The cabinet’s construction from
UV impermeable 10 mm acrylic also serves as an
effective barrier against radioactive isotopes, allowing
the user to work in complete safety with high energy
β-emitters such as 32P. A single white light bulb
illuminates the work area when the cabinet is in use.