5 collecting spectra and manipulating data, 1 overview, 2 collecting spectra – Ocean Optics DTS User Manual
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Polychromix DTS
TM
Near-Infrared Spectrometer
5-1
Operator’s Manual
Document Number 04-91-0201-00 Rev A
5 Collecting Spectra
and Manipulating
Data
5.1
Overview
This chapter demonstrates how the operator uses the Polychromix
SpectralCode application software program to collect spectra, manipulate
data, and store and retrieve data. The material in this chapter should be used
in conjunction with Chapter 6, which describes sample handling and related
issues.
NOTE: The general approach for collecting spectra and manipulating
data using SpectralCode is identical for all types of data (absorbance,
transmittance and reflectance). For the sake of brevity, only the
collection and processing of transmittance spectra are presented.
5.2
Collecting Spectra
5.2.1
Collecting the Background Spectrum
To Collect the Background Spectrum:
a) Place the blank in the sample compartment (e.g. an empty cuvette)
b) Press the
button
c) Press the
button to collect the spectrum (Figure 5-1).