YSI APPENDIX F - Manual (Page 2)

Quick Start Guide to Drinking Water Studies Appendix F
Setting up DW Sondes For Drinking Water Studies:
• After unpacking the instrument and the flow cell, install all sensors EXCEPT free chlorine as
described in Section 2.3 of the 6-series drinking water manual. Leave the port plug installed in
the “DO” port of the sonde. The chlorine probe will be installed in this port at a later time.
• Access the Advanced|Setup menu of the sonde and make certain that the “Sample and Hold”
feature is INACTIVE.
• Calibrate all sensors EXCEPT free chlorine according to standard procedures described in Section
2.5 of the 6-series drinking water sonde manual. Use the calibration vessel supplied with the
sonde to hold the calibration reagents.
CAUTION: Make certain that the free chlorine sensor is not present in the sonde
bulkhead when calibrating other sensors.
• Install the membrane for the free chlorine probe as described in Attachment 1 of this document
and in Section 2.3 of the DW sonde manual. Proceed IMMEDIATELY to the next step to avoid
the drying out of the free chlorine membrane and electrodes or store the probe in a beaker of tap
water (not distilled or deionized) until proceeding to the next step.
• Now you MUST activate the sensor according to the following instructions:
• Prepare a solution of free chlorine by diluting commercial bleach by a factor of 200. For
example, add 1 mL of commercial bleach to 200 mL of water (purified or tap).
• Place the sensor end of the probe in the bleach solution and leave for approximately 60
seconds.
• After 60 seconds in the bleach solution, remove the sensor and rinse with purified water.
CAUTION: Use of more concentrated bleach solutions and/or exposure times
longer than 60 seconds will cause a significant increase in the sensor break-in
period and, in extreme cases, may cause damage to the sensor.
• Remove the port plug from the “DO” port in the sonde bulkhead and install the free chlorine probe
according to the instructions in Section 2.3 of the manual. Be careful not to touch or damage the
membrane.
CAUTION: The sensor should either be installed in the sonde bulkhead and immersed
in water in the flow cell (see below) within 5 minutes after the activation process or the
probe itself should be placed in a beaker of tap water. It is important that the probe
does not dry out after activation
• Following the instructions in the flow cell kit supplied with your DW sonde, install the flow cell
on to the sonde. Be sure the proper O-ring is in place on the sonde above the threads of the probe
compartment.
• Attach a piece of tubing between your drinking water source and the bottom connector of the flow
cell and another piece of tubing between the top connector of the flow cell and your drain. Open
the water valve to allow a flow of 500-1000 mL per minute(minimum 500 mL/min). See picture
below for a typical laboratory setup.
YSI Incorporated Drinking Water Monitoring Systems Operations Manual F-2