Luminex MasterPlex QT Tutorial Manual User Manual

Page 25

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25 Master

Plex

QT


Note if you click on a different analyte, those standard concentration values are still zero.


You will need to repeat this procedure for each analyte. Note that some kit
manufacturers use the same starting concentration for all of the analytes in an assay. In
this case, you can select in the Auto Fill dialog box the Fill in for all bead sets option,
and it will use that starting concentration for all the analytes. For this exercise, please fill
in the starting concentration for each analyte before proceeding:

• IL-10 = 19,300 pg/mL
• IL-2 = 20,400 pg/mL
• IFN gamma = 5200 pg/mL
• IL-4 = 21,200 pg/mL


You can confirm the standard values by selecting Standard/Independent Values from
the pull-down pick-list, then clicking on each bead region in the Analytes list.

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