Luminex MasterPlex QT Tutorial Manual - Manual (Page 7)

http://www.miraibio.com 7 Master Plex QT
Also note the black borders that show how the wells are grouped. Please note that
MasterPlex QT treats standard wells in a single group differently than background wells,
unknown wells or control wells grouped together in a single group:
• When standard wells are placed in one group, MasterPlex QT will use data from
that group to create a standard curve for each analyte. If you have replicate
standard wells, MasterPlex QT will determine which wells are replicates by
comparing their standard/known concentration values. For replicate standards,
you can choose to calculate the standard curves by either using the average values
for the replicate wells, or by using the individual values.
• When background, unknown or control wells are grouped together, you can
instruct MasterPlex QT to treat each group as a set of replicate wells, and
calculate the mean, standard deviation and %CV for each group when you
calculate your unknown concentrations.
In the above picture, there is one group of standard wells, and there are seven different
standard wells in that group. In this example, there are no replicate standards. There are
three groups of unknown wells, each with 6 replicates, and one group of control wells,
also with six replicates.
Before we calculate the unknown concentrations, we should select the model equation we
wish to use. To assign the model equation, right-click with your mouse on one of the
wells in the standard group, and select Assign Model Equation, as shown below.