Multichannel Systems MEA1060-Up Manual - Manual (Page 17)

Operating the MEA Amplifier
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5.4 Stimulation
Recommended stimulus protocols for MEA electrodes
MCS recommends to use biphasic pulses only, with negative phase first. The duration of the
phases should be 100 μs each and the amplitude 100 mV to 3000 mV. (See also Potter, S. M.,
Wagenaar, D. A. and DeMarse, T. B. (2005). Closing the Loop: Stimulation Feedback Systems
for Embodied MEA Cultures. Advances in Network Electrophysiology Using Multi-Electrode
Arrays. M. Taketani and M. Baudry, Springer; Wagenaar, D. A., Madhavan, R., Pine, J. and
Potter, S. M. (2005). "Controlling bursting in cortical cultures with closed-loop multi-electrode
stimulation." J Neurosci 25(3): 680-8.)
Stimulation with MEA electrodes
You can use any MEA electrode for stimulation. Simply connect the output channel(s) of your
stimulus generator to the input socket(s) of any desired electrode(s).
You should use the slide switches for disconnecting stimulating electrodes from the amplifier.
5.5 Service and Maintenance
You should clean the contact pins of the amplifier with a soft tissue and pure (100 %) alcohol
from time to time, especially if you have problems with the noise level.