Multichannel Systems PPS2 Manual - Manual (Page 19)

PPS2 Manual
17
3 Application Example: MEA2100-System
The peristaltic perfusion PPS2-System consists of two peristaltic pumps. In this application
example, one is connected to a reservoir for ringer solution and pumps solution through
a perfusion heating PH01 to a MEA recording chamber inside a MEA2100 headstage.
The PH01 is connected to a temperature controller TC01/02 and heats the solution to a set
value. A cannula is connected to the second pump, which removes the ringer from the MEA
chamber and pumps it into a waste bottle. PH01 and cannula are positioned by magnetic
perfusion holders MPH. The MPHs connect both perfusion in- and outlet to the ground
of the recording system, via the metal plate in the front of the MEA2100 recording system.
3.1 Setup
1. Provide a power supply in the immediate vicinity of the installation site.
2. Place all devices on a stable and dry surface, where the air can circulate freely and the devices
are not exposed to direct sunlight. Take care not to position the PPS2 and fluid reservoirs
directly above the recording system, to prevent damage by accidentally spilled liquid.
3. Set up the computer with installed PPS2 program and connect the PPS2 with an USB cable.
4. Set up the MEA2100 system as described in the respective manual.
5. Connect the PH01 via tube to the inlet (left) peristaltic pump of the PPS2 and the bottle
with ringer solution.
6. Connect the outlet tube to the outlet (right) peristaltic pump and the waste bottle.
7. Add a hose clamp to the inlet and the outlet tube each.
8. Connect the ground of the PPS2 to the ground on the interface board of the MEA2100,
if necessary.