Multichannel Systems PPS2 Manual - Manual (Page 7)

PPS2 Manual
5
2.2 Setting Up and Connecting the PPS2 Pump
Warning: Do not start the perfusion until you have double-checked that the perfusion lines
are set up properly and that the inflow and outflow rate are matching. Spilled liquid may
irreversibly damage electronic instruments.
The PPS2 consists of two independent peristaltic pumps, which both are rotating
counterclockwise. The left one is intended for perfusion inlet, the right one for perfusion
outlet. It is of course also possible to use both pumps as independent perfusion inlet pumps,
and to use for example a vacuum pump for suction. This manual will focus on the intended
function as inlet and outlet pumps. Each pump cycle contains a droplet isolator. The outlet
pump is additionally equipped with a bubble detector.
Connecting the Tube Set
The following tubes are used:
 Identical Pharmed ® BPT (Saint–Gobain) tubes with an inner diameter of 1.65 mm
and an outer diameter of 3.35 mm around the pump revolver and for the connection
between outlet droplet isolator chamber and outlet pump, where the sensor of the
bubble detector is located.
 A PVC tube with larger inner diameter is used for the tube to the waste bottle.
 Silicon tubing is used for all other connections.
The specified tubing must be used for the pump revolvers to ensure that the volumes set
in the software are correct. Other tubing can be used for all other connections as well.
Sets of ten tubes for replacement are available from Multi Channel Systems (For example:
PPRT1.65-10 tubes: Pharmed ® BPT, ID: 1.65 mm, OD: 3.35 mm). Additionally you can order
a replacement set for all fluidic components PPS2-Set-F. Please see section “Ordering
Information” in the Appendix.