5 selecting oocytes for injection – Multichannel Systems Roboinject Manual User Manual

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Injection

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4.5 Selecting Oocytes for Injection

The next step after definition of the injection samples is the allocation of the sample(s)
to the respective wells.

Click the "Void Well" icon

to exclude certain wells from injection. The deselected wells

will be crossed out. Click the "Clear" icon

to set back the virtual well plate to the origin,

non-selected status.

First, please select an injection sample.

To assign a sample to a well, please click onto the colored tube on the icon first, and after that
on the well, you want to assign to the sample. The color of the well immediately changes to the
symbol color of the sample tube.

Click on the row letters A to H to assign single rows or on the column numbers 1 to 12 to assign
single columns.

If you want to select all wells, click on the Roboinject icon

on the upper left corner of the

virtual well plate.

If you like to apply a second sample, please proceed with the next sample. Again, the wells change
their color to the sample color when you select them. If you select wells already selected for
injection with a different sample the well will be divided into two halves with different colors.
Theoretically you can inject a single cell with four different compounds ending up with a virtual
well split into four quarters with the four different sample colors.

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