Next Advance Gold 24-AU - Manual (Page 4)

Operator’s Manual for the Bullet Blender
®
Gold
Example:
The figure above shows the Bullet
Blender
Gold set to run for 4 minutes at
a speed of 8, after running for 2
minutes. To operate, press the
“START” button. After 15 seconds, the
LED light by the number 15 will light
up. After 30 seconds, the second LED
light by the number 30 will light up.
And so on. At the settings shown
above, after 4 minutes, the Bullet
Blender
Gold will stop.
To use 4° C cooling, fill the dry ice
cooling compartment with at least 2 lbs
of dry ice. Use cryogenic gloves when
handling dry ice. Do not use the inner
portion of the dry ice cooling
compartment to transport the ice. Do
not fill the dry ice cooling compartment
with water ice. Make sure that the dry
ice cooling compartment lid is properly
sealed. To pre-cool the instrument, after
filling the compartment with dry ice,
run the instrument for 5 minutes or until
the “4°C” light comes on with no tubes
inside.
To use Air cooling™, where
ambient air is drawn into the
instrument, operate the Bullet Blender
Gold with the dry ice compartment
open. Air Cooling is most effective
when the instrument is operated in a
4°C environment.
PROTOCOLS AND SAMPLE
SETTINGS
The following ratio should be used
as a guideline for determining the
amount of beads and buffer to use given
a certain sample size - 1 volume/mass
of tissue: 1 volume of beads : 2
volumes of buffer. For more specific
information regarding the use of
various beads as well as specific
protocol information, please refer to our
website: www.nextadvance.com
As the tissue amount becomes
smaller, the above recommended ratio
may differ due to the limitations of
handling of the small volumes. We
recommend using a minimum of 25 μL
of buffer regardless of your sample
size.
The maximum sample mass is 300
μL per microcentrifuge tube in the
Bullet Blender
Gold, and the tube
should not be filled more than two-
thirds of the way (this includes sample,
beads, and buffer). The mechanics of
homogenization require empty space in
the tube.
Cutting the tissue into smaller
pieces will generally yield better
results. Tissue with a high aspect ratio
(long, thin strips) will homogenize
better than tissue that is round or cubic.
Do not operate the Bullet Blender
®
Gold using the same tubes for longer
than 15 minutes.
Protocols for many types of
samples are posted on our website, at
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