Eppendorf Eporator - Manual (Page 24)

Troubleshooting
Eppendorf Eporator®
English (EN)
24
• Deviating voltages during transformation: The voltage applied to the electroporation
cuvette ( act ) greatly differs from the set voltage ( set )
A too low resistance has several causes:
– The cells were washed and resuspended in a buffer with a too high ionic strength.
– The cells were not sufficiently cleaned during the preparation. After insufficient
washing, growth medium residue, which has been carried along, leaves behind
unwanted salts.
– Lysed cells are in the preparation. They contribute to the reduction of the resistance
of the medium.
– The salt concentration in the DNA preparation is too high.
6.2 Error messages

Quit all error messages with the Exit key.
If the proposed measures repeatedly fail to remedy the fault, contact your local
Eppendorf partner. The addresses of our distributors can be found on our
website www.eppendorf.com. The addresses of our sales offices can be found on
the second to last page of this operating manual.