Eppendorf 5424 R Centrifuge - Manual (Page 24)

5 Operation
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Centrifuge 5424 / 5424 R — Operating manual
2. Press the start/stop key to terminate the temperature control run early.
After the temperature control run is complete the centrifuge keeps the rotor chamber, with the
centrifuge lid closed, at the set temperature, if the temperature is below the ambient temperature.
Irrespective of the target temperature, however, this continuous cooling does not go below 4°C to
prevent the rotor chamber from freezing.
5.5.5 Continuous cooling
When the rotor is stopped the rotor chamber is kept at the target temperature when the following
prerequisites are met:
• The centrifuge is switched on.
• The centrifuge lid is closed.
• The target temperature is below the ambient temperature.
During continuous cooling the following applies:
• The target temperature is displayed.
• Irrespective of the target temperature, continuous cooling does not go below 4°C to prevent
the rotor chamber and the samples from freezing and increased condensation in the device.
• Because the rotor does not rotate during this process the temperature adjustment is slower.
To end continuous cooling, open the centrifuge lid.
If the centrifuge is not used for more than 8 hours, the continuous cooling is switched off
automatically. This protects against ice formation in the rotor chamber and in the tubes as well as
increased condensation in the device.
The device then switches to standby mode. The EP logo appears in the display.
With fast temp you can quickly reach the desired temperature again (see p. 23).
You can change continuous cooling to endless operation. To do so, in the device menu under
TEMP enable the 'oo' (see p. 20) option. Note that this may reduce the service life of the
compressor.
5.6 Centrifuging
Before using the Centrifuge 5424 / 5424 R for the first time, familiarize yourself with the operating
controls and the display (see Overview of operating controls on p. 18).
Each of the centrifuging variants described here must be preceded by the preparation described
above (see Preparing for centrifugation on p. 21).
Only 5424 R: Please also note the instructions on cooling (see p. 23).
CAUTION!
Risk from incorrectly-loaded rotors and damaged/overloaded tubes!
 Before commencing centrifugation, follow the safety instructions relating to hazards from
asymmetrically loaded and/or overloaded rotors and from overloaded, damaged and/or open
tubes (see Warnings for intended use on p. 13).
CAUTION!
Risk of injury from improperly attached rotors and rotor lids.
 Centrifuge only with the rotor and rotor lid firmly tightened.
 If unusual noises occur when the centrifuge starts, the rotor or the rotor lid may not be
properly secured. Stop centrifugation immediately by pressing the start/stop key.
EN
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