Eppendorf 5424 R Centrifuge - Manual (Page 22)

5 Operation
22
Centrifuge 5424 / 5424 R — Operating manual
To load the rotor, proceed as follows:
1. Check the maximum load (adapter, tube and content) per rotor bore.
The information about this can be found on every rotor and in this operating manual
(see Rotors on p. 10).
2. Load rotors and adapters only with the tubes intended for them.
3. Insert tubes opposite each other in pairs into the rotor bores. For symmetrical loading, tubes
that are opposite each other must be of the same type and contain the same filling quantity.
In order to minimize weight differences between filled sample tubes, we recommend taring
with a scale. This will reduce wear on the drive and cut running noise.
4. Attach and tighten rotor lid.
CAUTION!
Risk from damaged or overloaded tubes!
 When loading the rotor, note the safety instructions with regard to hazards from overloaded or
damaged tubes(see Warnings for intended use on p. 13)
Hint!
Rotor lid!
• Fixed-angle rotors may only be operated with the appropriate rotor lid in each case. This is
clearly shown by the identical rotor name labeling on the rotor and on the rotor lid.
• To carry out an aerosol-tight centrifugation, an aerosol-tight rotor (marking: red ring) and the
corresponding aerosol-tight rotor lid (marking: aerosol-tight and red lid screw) must be
used.
Hint!
With the rotors FA-45-24-11 and FA-45-24-11-Special centrifugation is also possible without a rotor lid.
Please also note:
• The tube lids must be closed securely.
• The rotors are not aerosol-tight without rotor lid.
• The centrifugation is slightly louder.
• Spin columns must always be centrifuged with a rotor lid.
Hint!
Spin columns
For centrifuging spin columns in the rotor F-45-18-11-Kit, you can leave the tube lids open.
However, this is possible only using the tubes provided for this purpose by kit manufacturers. For
reliable centrifugation, you must lean the open tube lids against the edge of the rotor. Ensure that
this does not involve the lids projecting beyond the edge of the rotor, and then put on the
associated rotor lid.
EN
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