Eppendorf 5424 R Centrifuge - Manual (Page 15)

15
3 Safety
Centrifuge 5424 / 5424 R — Operating manual
3.5.4 Extreme strain on the centrifuging tubes
CAUTION!
Risk of injury from asymmetric loading of rotors.
 Load rotors symmetrically with identical tubes.
 Only load adapters with suitable tubes.
 Always use tubes of the same type (weight, material/density and volume).
 Check symmetric loading by balancing the adapters and tubes used with scales.
CAUTION!
Risk of injury from overloaded rotor.
The Centrifuge 5424 / 5424 R is designed for the centrifugation of material with a max. density of
1.2 g/mL at maximum speed and volume and/or load.
 Observe the information on each rotor relating to maximum load (adapter, tube and contents)
per rotor bore and make sure it is not exceeded.
NOTICE!
Damage to rotors from aggressive chemicals.
Rotors are high-quality components which withstand extreme stresses. This stability can be
impaired by aggressive chemicals.
 Avoid the use of aggressive chemicals, including strong and weak alkali, strong acids,
solutions with mercury, copper and other heavy metal ions, halogenated hydrocarbons,
concentrated saline solutions and phenol.
 If the rotor is contaminated by aggressive chemicals, clean it immediately using a neutral
cleaning agent. This applies to the rotor bores in particular.
CAUTION!
Risk of injury from overloaded tubes.
 Note the loading limits specified by the tube manufacturer.
 Only use tubes which are approved by the manufacturer for the required rcf.
NOTICE!
Risk from damaged tubes.
Damaged tubes must not be used, as this could cause further damage to the device and the
accessories and loss of the samples.
 Before use, carry out a visual check of all tubes for any damage.
NOTICE!
Risk from open tube lids.
Open tube lids can break off during centrifugation and cause damage to the rotor as well as to
the centrifuge.
 Carefully close all tube lids before centrifuging.
Exception: Note the information on the centrifugation of spin columns in the rotor F-45-18-11-Kit
(see Load the rotor on p. 21).
NOTICE!
Hazard to plastic tubes from organic solvents.
When using organic solvents (e.g. phenol, chloroform) the density of plastic tubes is reduced,
i.e., the tubes could get damaged.
 Follow the manufacturer's information about the chemical resistance of tubes.
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