Eppendorf 5424 R Centrifuge - Manual (Page 25)

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5 Operation
Centrifuge 5424 / 5424 R — Operating manual
5.6.1 Centrifuging with time preset
Perform the following steps in the sequence described.
1. Use time to set the run time.
2. Only 5424 R: Use temp to set the temperature.
3. Use speed to set the g-force (rcf) or speed (rpm).
4. Press start/stop to start centrifuging.
During centrifugation
• In the display flashes while the rotor is running.
• Only 5424 R: The actual temperature is displayed.
• The fast temp (only 5424 R), open, short as well as the device menu are blocked during
centrifugation.
• During the run you can modify the total run time, the temperature (only 5424 R), the speed
and the rpm/rcf display.
The values flash in the display during the change. The new parameters are adopted
immediately. When the time is changed during a run, the time which has already elapsed
is taken into account. Note that the shortest new total run time which can be set is the time
which has already elapsed plus 2 minutes.
• You can also terminate the centrifugation before the set run time has elapsed by pressing
the start/stop key.
End of centrifugation
• After completion of the set time, the centrifuge stops automatically. During braking the
elapsed centrifugation time is displayed flashing. When the rotor stops a signal tone is
sounded.
• Only 5424: The centrifuge lid opens automatically. The display shows the symbol .
• Only 5424 R: The centrifuge lid remains closed to maintain the sample temperature. You
can open it by pressing the open key.
5. Remove centrifuge contents.
5.6.2 Centrifuging in continuous operation
Perform the following steps in the sequence described.
1. Use time to set the continuous run.
The continuous run function can be set above 9:59 h or below 30 s. The timer shows oo to
indicate continuous operation.
2. Only 5424 R: Use the temp arrow keys to adjust the temperature.
3. Use speed to set the g-force (rcf) or speed (rpm).
4. Press start/stop to start centrifuging.
In the display flashes while the rotor is running.
Time is counted upwards, first in 30-second increments (5424) or one-second-increments
(5424 R) and then in minute increments from ten minutes.
5. Press start/stop to end centrifuging after the desired time period.
• During the braking process, centrifuging duration flashes in the display.
• When the rotor stops a signal tone is sounded.
• Only 5424: The centrifuge lid opens automatically. The display shows the symbol .
• Only 5424 R: The centrifuge lid remains closed to maintain the sample temperature. You
can open it by pressing the open key.
6. Remove centrifuge contents.
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