Eppendorf D30 BioPhotometer - Manual (Page 30)

Methods
Eppendorf BioPhotometer
® D30
English (EN)
30
You can create new methods in all folders using <New Method>.
In Favorites, you can create your own folders (e.g., to allocate folders to specific people), and rename and
delete the folders.
Tab. 6-2: Softkeys in method selection
Copied or cut methods can be added to a different folder under Favorites, or added to the original folder
under a new name. Use the cursor keys to navigate to the
Methods column of the desired folder and press
[paste] for adding the method.
6.2 Photometry method description
The preprogrammed methods and method templates are described in this section.
6.2.1 Absorbance method group
Single λ
• Absorbance measurement on a wavelength.
• No subsequent evaluation.
6.2.2 Routine method group
The methods for the Routine group are preprogrammed as fixed methods. Therefore, a new method name
is required after the method parameters in the fixed preprogrammed methods have been modified.
Nucleic acids
• Determination of the concentration of nucleic acids through measurement at 260 nm and evaluation via
factor.
• Various nucleic acid methods, such as dsDNA or RNA, are preprogrammed. The parameters vary
according to the factor.
• Preprogrammed method for microliter cuvettes: Measuring DNA in sample volumes within the
microliter range with 1 mm light path (with microliter cuvettes as Eppendorf μCuvette G1.0 or Hellma ®
TrayCell).
• Additional information on the purity of the measured nucleic acid: Ratios A260/A280, ratios A260/A230,
restricted absorbance wavelength spectrum of nucleic acid (with 3 nm distances), absorbance of the
background wavelength (preset: 320 nm; the absorbance of the pure nucleic acid should be close to
zero here).
• Partial turbidity correction can be performed via the Background parameter.
• Concentrations can be converted to molar concentrations and (after the sample volume has been
entered) to nucleic acid quantities ( process results method step).
[Cut] and [Paste] Cut and paste methods.
[Copy] and [Paste] Copy and paste methods.
[Delete] Delete methods.
[Rename] Rename methods.