Eppendorf Pipette Reference 2 - Manual (Page 15)

Operation
Eppendorf Reference ® 2
English (EN)
15
4 Operation
4.1 Setting the volume (only Reference 2 variable)
4.2 Using pipette tips
The liquid to be dispensed is aspirated into pipette tips. We recommend using epT.I.P.S. epT.I.P.S. pipette
tips, which are available with various degrees of purity, with and without filters and as special tips. We
recommend using special epT.I.P.S.
LoRetention tips with liquids that have a lower surface tension than
water (e.g., due to a wetting agent in the liquid).
Pipette tips are for single use only.
 To set the volume, hold the volume locking button pressed down
and turn the control button.
We recommend adjusting the volume setting from a higher value to a
lower value. If required, turn beyond the required value and then
back again.
The height of the control button changes as the volume is adjusted.
The volume is displayed up to 1000
μL in μL. For 2 mL to 10 mL
pipettes, the volume is displayed in mL.
The pipette is only functional if a pipette tip has been attached.
You can either attach the pipette tip by hand or directly insert
the end of the pipette into a tip held in the tip storage box. If
attaching a pipette tip by hand, it must be handled in a way that
prevents contamination and heating of the pipette tip.
The color code on the Reference 2 control button matches the
color of the epT.I.P.S. trays
 If you use pipette tips without filter, insert the protection
filter in the 2
mL to 10 mL pipette.
 Fit the suitable pipette tip(s) on the tip cone, applying light
pressure.
The pipette tip sits firmly on the tip cone when it spring loads
(exceptions: no spring loading for 2
mL to 10 mL
single-channel pipettes.)
0 0 0 0
10-100 µL
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