Leica Biosystems M80 B - Manual (Page 53)

Leica M series User Manual 53
Calculating the Total Magnification and Field of View Diameter
Parameter
MO Magnification of objective
ME Magnification of eyepiece
z Magnification changer position
q Tube factor, e.g. 1.5× for coaxial incident light, 1.6× for 45°
ErgoTube™
r Factor 1.25× if the planachromatic and planapochromatic
objectives of the MZ125/MZ16 are used on the MS5, MZ6,
MZ75 or MZ95
NFOV Field number of the eyepiece. Field numbers are printed on the
eyepieces: 10Ч = 21, 16Ч = 14, 25Ч = 9.5, 40Ч = 6.
Example
MO 1× objective
ME 25×/9.5 eyepiece
z Zoom position 4
q Coaxial incident light 1.5×, tube factor
r Factor 1.25×
Calculation example: magnification in the binocular tube
MTOT VIS = MO × ME × z × q × r
or
1 Ч 25 Ч 4 Ч 1.5 Ч 1.25 = 187.5Ч
Calculation example: field of view diameter in the specimen
∅ OF:
NFOV
MO × z ×q Чr