LaMotte Nitrate-Nitrogen Low Range Enzyme Kit User Manual
Page 5
Pro ce dure
High Range (0.2 – 1.0 ppm NO
3
_N)
1.
Firmly tap the capped Enzyme Vial (8136) three times on a hard
surface. This will ensure that reagents are at the bottom of the vial.
2.
Use the 1 mL pipet (0354) to add 2 mL of Nitrate Buffer (8139).
3.
Use the 0.5 mL pipet (0353) to add 0.5 mL of the sample water to
the Enzyme vial.
4.
Use a plain pipet (0352) to add Deionized Water (5115) until the
vial is filled to the 5 mL line.
5.
Cap the vial. Mix by inverting gently 3 times. Repeat the mixing
procedure at 5 minutes, 10 minutes and 15 minutes.The total
reaction time is 15 minutes.
6.
Add 5 drops Hydrochloric Acid, 1N (6130). Cap and mix.
7.
Add 1 Nitrate Color Tablet (8140A). Cap the vial and mix by gently
inverting until the tablet disintegrates.
8.
Wait 5 minutes.
9.
Remove the cap. Place the vial on the white area of the Nitrate-N
High Range Color Chart (8137HR-CC). Looking down through the
tube, compare the color of the solution to the color chart.
10.
Record the result as ppm Nitrate-N (NO
3
_N). To convert the result
to Nitrate (NO
3
) multiply by 4.4.
NOTE:
Salinity will reduce the enzyme effectiveness and result in a decrease
of color development.
NOTE:
For the most ac cu rate re sults use lab o ra tory-grade pipets for
mea sur ing sam ple wa ter and re agents.