Bio-Rad MicroPulser™ Electroporator - Manual (Page 13)

Fig. 5. MicroPulser control panel.
Section 4
MicroPulser Operating Instructions
Refer to Figure 1 for a view of the components of the MicroPulser system and to Figure 5
for a definition of the buttons and LEDs.
4.1 Setting Up The MicroPulser System
1. Connect the black power cord to the rear panel of the MicroPulser Pulse Generator
Module. Plug the cord into a wall outlet or power strip.
2. Pull down the fold-down foot on the underside of the MicroPulser. Insert this foot into the
track on the base of the shocking chamber. Insert the shocking chamber slide into the
shocking chamber.
3. Connect the leads from the shocking chamber to the output jacks on the front panel of the
MicroPulser; polarity is not important to the electroporation process.
4. Turn on the apparatus using the power switch on the right rear panel. The light emitting
diode (LED) display should illuminate and read “Ec1” and the LED next to the Bacteria
Settings should be illuminated.
4.2 Operation of the MicroPulser
1. Selecting Pre-Programmed Settings
The MicroPulser is pre-programmed with settings for electroporation of a number of
commonly used organisms. Included under the Bacteria Settings program are the following:
Mnemonic Organism Parameters
Voltage Number of Time constant
(kV) pulses (msec)
Ec1 E. coli (0.1 cm cuvette) 1.8 1 -
Ec2 E. coli (0.2 cm cuvette) 2.5 1 -
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