Bio-Rad MicroPulser™ Electroporator - Manual (Page 26)

25. Siguret, V., Ribba, A.-S., Cherel, G., Meyer, D., and Pieru, G., Effect of plasmid size on transfor-
mation efficiency by electrtoporation of Escherichia coli DH5 α, Biotechniques, 16, 422 (1994).
26. Simon, D. and Ferretti, J.J., Electrotransformation of Streptococcus pyogenes with plasmid and
linear DNA, FEMS Micobiol. Lett., 82, 219 (1991).
27. Somkuti, G.A. and Steinberg, Genetic transformation of Streptococcus thermophilus by electro-
poration, Biochimie, 70, 579 (1988).
28. Summers, D. K., and Withers, H. L., Electrotransfer: direct transfer of bacterial plasmid DNA by
electroporation, Nuc. Acids Res., 18, 2192 (1990).
29. Taketo, A., DNA transfection of Escherichia coli by electroporation, Biochim. Biophys. Acta, 949,
318 (1988).
30. Taketo, A., RNA transfection of Escherichia coli by electroporation, Biochim. Biophys. Acta, 1007,
127 (1989).
31. Trevors, J.T., Chassy, B.M., Dower, W.J., and Blaschek, H.P., Electrotransformation of bacteria by
plasmid DNA, in Guide to Electroporation and Electrofusion, Chang, D. C., Chassy, B. M.,
Saunders, J. A., and Sowers, A. E. (eds.), Academic Press Inc., 265, San Diego (1992).
32. Willson, T. A., and Gough, N. M., High voltage E. coli electro-transformation with DNA follow-
ing ligation, Nuc. Acids Res., 16, 11820 (1988).
33. Zabarovsky, E. R., and Winberg, G., High efficiency electroporation of ligated DNA into bacteria,
Nuc. Acids Res., 18, 5912 (1990).
Appendix II
Troubleshooting Guide for the Micropulser
Operational Problem Possible cause and solution
1. Display does not light Power is not supplied to electronics.
when unit is turned on. Check power cord and wall outlet power source.
Check that power switch is on.
Check/replace fuse. Two 2 A, 250 V Type T fuses
are located on the back of the instrument immedi-
ately above the power cord.
2. When the buttons are pressed, No pulse delivery.
the unit does not indicate “PLS”. Pulse button is not depressed hard enough
Verify instrument operation by the following tests:
Turn on the power switch.
Verify the LED next to “Bacteria” is illuminated.
Press the “Settings” button.
Verify the LED next to “Fungi” is illuminated.
Press the “Settings” button again.
Verify the LED next to “Manual” is illuminated.
Press the “Settings” button again.
The LED next to “Bacteria” should be lit.
Press the “Raise” button several times to
change the display from “Ec1” to “Ec3”.
Press the “Raise” and “Lower” buttons simul-
taneaously to verify the display reads “3.00”
volts.
Press the “Pulse” button until “PLS” is dis-
played; a beep tone should be heard within
6 sec.
Press the “Measurements” button.
Verify the “Actual kV” LED illuminates.
Verify the display LED shows a reading
between “2.95” and “3.05”.
Press the “Measurements” button again.
Verify the “Time ms” LED illuminates.
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