Bio-Rad PROTEAN Plus Multi-Casting Chamber - Manual (Page 4)

2
Fig. 1. PROTEAN plus Multi-Casting Chamber.
Section 2
Preparing the Multi-Casting Chamber
2.1 Loading the Chamber
1. Loosen the thumb screws, remove the sealing plate, and place the open casting chamber
face up on the benchtop. The thumbscrew holes should face the ceiling.
2. Start by placing a separation sheet into the chamber so that it seats at the bottom. (Be
sure to remove the protective film from the separation sheet prior to use.)
3. Place the PROTEAN plus hinged spacer plate into the casting chamber body. It is easiest
to insert the plate at a slight angle, hinged side first. Lower the other side into the chamber
so the plate lays flat.
4. Place a separation sheet on top of the hinged spacer plate.
5. Repeat steps 3–4 until you have prepared the desired number of gel sandwiches.
6. Take up the remaining space in the multi-casting chamber with acrylic blocks and
separation sheets so that the sandwiches will be held firmly in position when the sealing
plate is in place.
Note: To insure a good seal, the entire stack should be made as flush as possible to the front
of the multi-casting chamber, and not extend beyond it. If you overfill the multi-casting chamber,
the sealing plate will not be able to create a good seal with the gasket. This causes the monomer
solution to leak during pouring, and glass plates within the stack may break.
Gasket
Thumb screws
Sealing plate
Inlet port with Luer connector
Casting
chamber